Evaluating LoVo Cell Viability

To assess the cell viability of LoVo cells, the MTT [3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium-bromide] assay was performed. A 96-well plate was seeded with 1 x 10⁴ parental LoVo cells and 1 x 10⁴ OXA-R LoVo cells, and the cells were exposed to various drug doses. Oxaliplatin was treated to parental LoVo and OXA-R LoVo cells at 1, 5, 10, 15, 20, 25, 30, 40, 50, 60, 70, and 80 µM for 24 hours, After the treatment time, DMEM was removed from the cells and washed with PBS. Each well received 20 μl (5 mg/mL) of MTT, which was then left to incubate for 4 hours. A microplate reader was used to detect the absorbance at 570 nm after dissolving MTT formazan crystals in 200 μl of DMSO. Platycodin D (purity ≥98%, Sigma, USA) was dissolved in DMSO.