Collagen-Chitosan Hydrogel Scaffold for Tissue Engineering

Bovine Collagen type I, 8 wt.% aqueous solution (Coll, Collado s.r.o., Brno, Czech Republic), chitosan from shrimp shells, 70% DDA, low viscosity (Chit, Sigma-Aldrich, Darmstadt, Germany), calcium salt of oxidized cellulose–degree of oxidation 16–24% and M_n = 350 kg/mol (CaOC, Synthesia, Pardubice, Czech Republic), acetic acid (99%, Penta s.r.o, Chrudim, Czech Republic), poly(ε-caprolactone) (PCL, 80 kg/mol), gelatin (Gel, Type B, Bioreagent, powder from bovine skin), N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), 98% dopamine hydrochloride, tris (hydroxymethyl) aminomethane hydrochloride, ethanol p.a. 99.8%, sodium phosphate dibasic for molecular biology (≥ 98,5%), sodium chloride, calcium chloride, sodium phosphate dibasic dodecahydrate (Na₂HPO₄ ·12H₂O), potassium dihydrogen phosphate (KH₂PO₄), potassium chloride (KCl), collagenase from Clostridium histolyticum, lysozyme human, the murine fibroblast cell lines 3T3-A31, Dulbecco's modified eagle medium DMEM (D6429), fetal bovine serum FBS (F7524), 2′,7′-bis (2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM), propidium iodide (P4864), (all from Sigma Aldrich, Darmstadt, Germany), penicillin/streptomycin (15140–122) and DiOC6(3) (D273), (Life Technologies, Eugene, OR, USA), octenidine solution (Octenisept®, Schülke, Germany), Butomidor^® inj. (butorphanol tartrate, Vétoquinol, Czech republic), Domitor^®, Medetomidine, Orion corporation, Finland) Propofol^® (Propofolum 1%, Fresenius Kabi Deutschland, Bad Homburg, Germany), Metacam^® (meloxicam, Boehringer Ingelheim Vetmedica, Ingelheim/Rhein, Germany), Enroxil^® (Enrofloxacin, Krka, Novo mesto, Slovenia) Betadine^®, (2.5% solution of povidone iodine, EGIS Pharmaceuticals PLC, Budapest, Hungary) were used as received without further purification.

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Kacvinská K., Pavliňáková V., Poláček P., Michlovská L., Blahnová V.H., Filová E., Knoz M., Lipový B., Holoubek J., Faldyna M., Pavlovský Z., Vícenová M., Cvanová M., Jarkovský J, & Vojtová L. (2023). Accelular nanofibrous bilayer scaffold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model affects inflammation and healing process. Journal of Nanobiotechnology, 21, 80.

Publication 2023

Acetic acid Aminomethane hydrochloride Bcecf am Betadine Bovine Butorphanol tartrate Calcium Calcium chloride Caoc Caprolactone Carboxyfluorescein Cell lines Chitosan Clostridium histolyticum collagenase Collagen type i Dioc6 Dopamine hydrochloride Eagle Enrofloxacin Ester Ethanol Fibroblast Gelatin Human Lysozyme Medetomidine Meloxicam Murine N hydroxysuccinimide Octenidine Octenisept Oxidized cellulose Penicillin Penta Pharmaceuticals Poly Potassium dihydrogen phosphate Povidone iodine Powder Propidium iodide Propofolum Rhein Skin Sodium chloride Sodium phosphate Streptomycin Tris Viscosity

Corresponding Organization :

Other organizations : Central European Institute of Technology, Brno University of Technology, Czech Academy of Sciences, Institute of Experimental Medicine, Masaryk University, University Hospital Brno

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Variable analysis

independent variables

Bovine Collagen type I, 8 wt.% aqueous solution (Coll, Collado s.r.o., Brno, Czech Republic)
Chitosan from shrimp shells, 70% DDA, low viscosity (Chit, Sigma-Aldrich, Darmstadt, Germany)
Calcium salt of oxidized cellulose–degree of oxidation 16–24% and Mn = 350 kg/mol (CaOC, Synthesia, Pardubice, Czech Republic)
Poly(ε-caprolactone) (PCL, 80 kg/mol)
Gelatin (Gel, Type B, Bioreagent, powder from bovine skin)
N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride (EDC)
N-hydroxysuccinimide (NHS)
Dopamine hydrochloride

dependent variables

Not explicitly mentioned

control variables

Acetic acid (99%, Penta s.r.o, Chrudim, Czech Republic)
Tris (hydroxymethyl) aminomethane hydrochloride
Ethanol p.a. 99.8%
Sodium phosphate dibasic for molecular biology (≥ 98,5%)
Sodium chloride
Calcium chloride
Sodium phosphate dibasic dodecahydrate (Na2HPO4 ·12H2O)
Potassium dihydrogen phosphate (KH2PO4)
Potassium chloride (KCl)
Collagenase from Clostridium histolyticum
Lysozyme human
Murine fibroblast cell lines 3T3-A31
Dulbecco's modified eagle medium DMEM (D6429)
Fetal bovine serum FBS (F7524)
2',7'-bis (2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM)
Propidium iodide (P4864)
Penicillin/streptomycin (15140–122)
DiOC6(3) (D273)
Octenidine solution (Octenisept®, Schülke, Germany)
Butomidor® inj. (butorphanol tartrate, Vétoquinol, Czech republic)
Domitor®, Medetomidine, Orion corporation, Finland
Propofol® (Propofolum 1%, Fresenius Kabi Deutschland, Bad Homburg, Germany)
Metacam® (meloxicam, Boehringer Ingelheim Vetmedica, Ingelheim/Rhein, Germany)
Enroxil® (Enrofloxacin, Krka, Novo mesto, Slovenia)
Betadine® (2.5% solution of povidone iodine, EGIS Pharmaceuticals PLC, Budapest, Hungary)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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