Five SNPs in the OXTR (rs53576, rs2254298, rs1042778, rs2268494, and rs2268490) gene were selected for inclusion in this study, which was particularly underscored in human research (Feldman et al., 2016 (link)). More detailed information about the SNPs is presented in Table 1 .
DNA was extracted from the peripheral venous blood of each freshman using the Trizol protocol. Genotyping was conducted by the matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometer on the MassARRAY® Analyzer 4 platform (Sequenom, San Diego, CA, USA). My-Sequenom online software Assay Design Suite v2.0 was applied to design probes and primers (Supplementary Material S2 ). For the genetic analyses, we employed SHEsisPlus (http://shesisplus.bio-x.cn/SHEsis.html ) to analyze allelic and genotypic distributions and the Hardy–Weinberg equilibrium (Shen et al., 2016 (link)). In this sample, the distribution of both genotypes showed no deviation from the Hardy–Weinberg equilibrium.
DNA was extracted from the peripheral venous blood of each freshman using the Trizol protocol. Genotyping was conducted by the matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometer on the MassARRAY® Analyzer 4 platform (Sequenom, San Diego, CA, USA). My-Sequenom online software Assay Design Suite v2.0 was applied to design probes and primers (
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