ChIP-qPCR Analysis of MICA Promoter

ChIP was conducted as previously described [14 (link)]. The ChIP-IT^® Express Enzymatic Magnetic Chromatin Immunoprecipitation Kit & Enzymatic Shearing Kit purchased from Active Motif (Carlsbad, CA, USA) was used for the ChIP assay. We designed seven pairs of qPCR primers in the human MICA transcript promoter region. Huh-7 cells were harvested after cisplatin was treated with a dose of 10 µM for 6 h. ChIP experimental procedures are performed according to the manufacturer’s instructions. Anti IRF1 antibody (ab243895, abcam, ChIP grade) was used in the experiment. The DNA collected by ChIP was detected by qPCR.