Levels of IL-6 and IL-12/23 p40 in the intestinal lavages and plasma were measured by a paramagnetic sphere-based xMAP technology (Luminex Corporation, Austin, TX, USA) with a Porcine ProcartaPlex kit (Affymetrix, Santa Clara, CA, USA) on the Bio-Plex Multi Array System (Bio-Rad, Hercules, TX, USA) and evaluated by Bio-Plex Manager 4.01 software (Bio-Rad), as described previously [75 (link)].
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