FITC-Dextran Bioavailability in Serum

Mice were administered with 100 μl of 100 mg/ml 70-kDa FITC-dextran (Sigma-Aldrich) in sterile water, and blood was collected 4 h before sacrifice (Wang et al., 2020b (link)). Serum was obtained by centrifugation (3,000 rpm, 10 min, 4°C). The concentration of FITC in serum was determined by SynergyH1 automatic microplate reader (Biotek) with excitation wavelength of 485 nm and emission wavelength of 528 nm (Pu et al., 2021 (link)).

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Wan F., Wang M., Zhong R., Chen L., Han H., Liu L., Zhao Y., Lv H., Hou F., Yi B, & Zhang H. (2022). Supplementation With Chinese Medicinal Plant Extracts From Lonicera hypoglauca and Scutellaria baicalensis Mitigates Colonic Inflammation by Regulating Oxidative Stress and Gut Microbiota in a Colitis Mouse Model. Frontiers in Cellular and Infection Microbiology, 11, 798052.

Publication 2021

70-kDa FITC-dextran SynergyH1 automatic microplate reader

Blood Centrifugation Fitc Fitc dextran Mice Serum Sterile

Corresponding Organization : Chinese Academy of Agricultural Sciences

Other organizations : Lanzhou University, Ministry of Agriculture and Rural Affairs, University of Liège, Gembloux Agro-Bio Tech

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Variable analysis

independent variables

Administration of 100 μl of 100 mg/ml 70-kDa FITC-dextran (Sigma-Aldrich) in sterile water

dependent variables

Concentration of FITC in serum

control variables

Blood collection time (4 h before sacrifice)
Serum preparation (centrifugation at 3,000 rpm, 10 min, 4°C)
Measurement of FITC concentration (excitation wavelength of 485 nm and emission wavelength of 528 nm)

controls

No positive or negative controls were explicitly mentioned.

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