Fourteen linear craniofacial measurements between landmarks were calculated using Dolphin Imaging software. These measurements include six standard measurements currently in use by the Craniofacial Mutant Mouse Resource of Jackson Laboratory; which are nasal bone length (landmark 1 to 2), nose length (landmark 1 to 3), inner canthal distance (landmark 14 to 15), skull width (landmark 26 to 27) and upper jaw length (landmark 1 to 22, 23). The Jackson lab skull height measurement was substituted with a cranial height measurement measured between pari (landmark 4) and the inferior portion of the spheno-occipital synchondrosis (landmark 30), due to the omission of the mandible in our study. Linear measurements were also calculated for frontal bone length (landmark 2 to 3), parietal bone length (landmark 3 to 4), zygomatic arch length (landmarks 12,13 to 24,25), anterior cranial base length (landmark 2 to 30) and posterior cranial base length (landmark 30 to 32). Linear distances of bilateral structures (upper jaw length and zygomatic arch length) were averaged from right and left measurements for each mouse. Data is presented as means +/− standard deviations. Statistical significance between measurements was established by the student’s t test.