Identifying HPV-Related Ciliated Carcinomas

As HPV-related carcinomas of the oropharynx can occasionally be ciliated and even spread to the neck as ciliated cystic metastases, HPV studies were performed on the ciliated MEC to exclude a ciliated HPV-related carcinoma.(11 (link), 12 (link)) Five-micrometer thick sections prepared from formalin-fixed and paraffin embedded tissue blocks. HPV testing consisted of p16 immunohistochemistry (clone INK4a; MTM Laboratories, Heidelberg, Germany) and HPV RNA in situ hybridization using the RNAscope HPV-HR18 Probe (Advanced Cell Diagnostics, Hayward, CA) which recognizes 18 high-risk HPV genotypes (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82) as previously detailed.(13 (link)) P16 staining in the nucleus and cytoplasm of 70% or more of the tumor cells was regarded as a positive stain. For p16 immunohistochemistry and RNA in situ hybridization, a known HPV-related oropharyngeal carcinoma served as a positive control.

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Bishop J.A., Cowan M.L., Shum C.H, & Westra W.H. (2018). MAML2 Rearrangements in Variant Forms of Mucoepidermoid Carcinoma: Ancillary Diagnostic Testing for the Ciliated and Warthin-Like Variants. The American journal of surgical pathology, 42(1), 130-136.

Publication 2017

RNAscope HPV-HR18 Probe

Carcinoma Cells Clone Cystic Cytoplasm Diagnostics Formalin Genotypes Immunohistochemistry In situ hybridization Metastases Neck Nucleus Oropharyngeal carcinoma Paraffin Stain Tissue Tumor

Corresponding Organization : Johns Hopkins University

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Variable analysis

independent variables

HPV testing

dependent variables

Presence of ciliated HPV-related carcinoma

control variables

Five-micrometer thick sections prepared from formalin-fixed and paraffin embedded tissue blocks
P16 immunohistochemistry (clone INK4a; MTM Laboratories, Heidelberg, Germany)
HPV RNA in situ hybridization using the RNAscope HPV-HR18 Probe (Advanced Cell Diagnostics, Hayward, CA) which recognizes 18 high-risk HPV genotypes (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82)

positive control

A known HPV-related oropharyngeal carcinoma

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