5’-bromo-2’-deoxyuridine (BrdU) detection of proliferation was performed as previously described (6 (link)). Briefly, mice were administered 0.8 mg of BrdU by i.p. injection, and maintained on BrdU in drinking water (0.8 mg/mL, plus 10% dextrose), ad libitum, from days 4-8 post-infection. Peritoneal cavity wash cells were stained for surface markers, and fixed using a BD Cytofix/Cytoperm solution, for 30 minutes at 4°C. Prior to pelleting the cells, BD Perm/Wash solution was added. Cells were then resuspended in 10% dimethyl sulfoxide (DMSO) in BD Perm/Wash solution and incubated for 10 minutes at 4°C. Cells were then fixed again with BD Cytofix/Cytoperm solution for 5 minutes at 4°C. DNase I in 1x Phosphate Buffered Saline (PBS) was then incubated on the cells for 1 hour at 37°C. The cells were then stained with FITC-conjugated anti-BrdU (Bu20a) diluted in BD Perm/Wash solution for 30 minutes at room temperature. Cells were then washed and analyzed as below.