Scoring and Analyzing AFLP Data in Tomato Cultivars

The scoring of AFLP data was done as 1 for the presence and 0 for the absence of each locus within the size range of 50–500 base pairs (bp). Genotypic diversity within each population was determined as described by Stoddart and Taylor (1988 (link)) followed by scaling it by the number of genotypes (g) (Grünwald et al. 2003 (link)). Nei’s gene diversity (Nei 1973 (link)), the number of polymorphic loci and genetic distance based on Nei’s (1978 (link)) unbiased measures of genetic distance were determined using POPGENE (v 1.32) (Yeh and Boyle 1997 ). UPGMA (unweighed pair group method with arithmetic mean) analysis used Nei’s unbiased measures of genetic distance for the construction of a dendrogram (NTSYSpc v 2.21m) (Rohlf 2009 ). Arlequin program version 3.1 (Excoffier et al. 2005 (link)) was used for analysis of molecular variance (AMOVA) among the populations obtained from the two tomato cultivars.