The protein expression of c-myc, Ras, activated caspase-3 and PARP-1 in tumor-bearing mice were evaluated by immunohistochemical analysis. Briefly, tumorous tissue was removed from tumor-bearing mice and cut into 5-mm-thick paraffin sections. The sections were deparaffinized and rehydrated according to standard protocols. The sections were then incubated with primary antibody (1:100 dilution; Abcam Trading Company, Ltd. Shanghai, China) at 4 °C overnight. Nonimmune (IgG) was used as a negative control, and antigenic sites were identified using an SP9000 Polymer Detection System and a 3,3’-diaminobenzidine kit (ZSGB-BIO, Beijing, China). The standard protocols were performed in accordance with approved guidelines [19 (link), 20 (link)].
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Immunohistochemical Evaluation of Tumor Markers
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Other organizations : Hainan Medical College Hospital
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Variable analysis
independent variables
- Tumor-bearing mice
- Protein expression of c-myc
- Protein expression of Ras
- Protein expression of activated caspase-3
- Protein expression of PARP-1
- Paraffin sections
- Incubation with primary antibody (1:100 dilution; Abcam Trading Company, Ltd. Shanghai, China) at 4 °C overnight
- Nonimmune (IgG) as a negative control
- Antigenic sites identified using an SP9000 Polymer Detection System and a 3,3'-diaminobenzidine kit (ZSGB-BIO, Beijing, China)
- Not specified
- Nonimmune (IgG)
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