Comprehensive Enzyme Substrate Assay

Tween 20, Pefabloc, para-nitrophenyl acetate (pNPA), para-nitrophenyl butyrate (pNPB), para-nitrophenyl palmytate (pNPP), para-nitrophenol (pNP), 2,2′-Azino-bis(3-ethylbenzothiazoline-6sulfonic acid) diammonium salt (ABTS), and alkaline lignin were purchased from Sigma Aldrich (France). AZCL-xylan (birchwood), AZCL-xyloglucan (tamarind), AZCL-HE Cellulose, AZCLarabinoxylan (wheat), AZCL-debranched arabinan, AZCL-Barley β-glucan, AZO-CM Cellulose, AZO-Avicel, AZO-α cellulose, AZO-Carob Galactomannan were purchased from Megazyme (Ireland).

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Ufarté L., Laville É., Lazuka A., Lajus S., Bouhajja E., Cecchini D.A., Rizzo A., Amblard E., Henrissat B., Lombard V., Terrapon N., Henrissat B., Cleret M., Morgavi D., Dumon C., Robe P., Klopp C., Bozonnet S., Hernandez‐Raquet G., & Laville É. (2024). Functional exploration of in vivo and in vitro lignocellulose-fed rumen bacterial microbiomes reveals novel enzymes involved in polysaccharide breakdown.

Publication 2024

Corresponding Organization : Centre National de la Recherche Scientifique

Other organizations : Architecture et Fonction des Macromolécules Biologiques, Aix-Marseille Université, VetAgro Sup, Université Clermont Auvergne, Givaudan (France), Genopole (France), Génétique Physiologie et Systèmes d'Elevage

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Variable analysis

independent variables

Tween 20
Pefabloc
Para-nitrophenyl acetate (pNPA)
Para-nitrophenyl butyrate (pNPB)
Para-nitrophenyl palmytate (pNPP)
AZCL-xylan (birchwood)
AZCL-xyloglucan (tamarind)
AZCL-HE Cellulose
AZCL-arabinoxylan (wheat)
AZCL-debranched arabinan
AZCL-Barley β-glucan
AZO-CM Cellulose
AZO-Avicel
AZO-α cellulose
AZO-Carob Galactomannan

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Para-nitrophenol (pNP)
2,2′-Azino-bis(3-ethylbenzothiazoline-6sulfonic acid) diammonium salt (ABTS)

negative controls

Alkaline lignin

Annotations

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