Working dilutions consisted 20% cremaphor (Sigma, St. Louis, USA), 20% dimethyl sulfoxide (Sigma, St. Louis, USA) and 60% phosphate-buffered saline (PBS). SP2509 was obtained from Med Chem Express (New Jersey, USA). Stock solutions were prepared by dissolving the components in the working dilutions with a concentration of 5 mg/ml.
Rats were separated randomly in three groups (n = 10 for each group), including sham-operated, SCI-only, and Add-SP2509 groups: one was treated with 25 mg/kg SP2509 immediately and 48 h after the wound was closed, while the other two groups were treated with 5 ml/kg working dilutions20 (link),44 (link).
Rats were separated randomly in three groups (n = 10 for each group), including sham-operated, SCI-only, and Add-SP2509 groups: one was treated with 25 mg/kg SP2509 immediately and 48 h after the wound was closed, while the other two groups were treated with 5 ml/kg working dilutions20 (link),44 (link).
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