Semi-quantitative PCR for Gene Expression

First-strand cDNAs were synthesized using the Prime Script™ RT Reagent Kit with gDNA Eraser (Takara, Kyoto, Japan). Semi-quantitative PCR reactions were performed using 2xTaq Plus Master Mix (Dye Plus) (Vazyme, Nanjing, China). The PCR conditions were as described previously [6 (link)]. Three biological repetitions were performed. The specific primers for the selected genes and the internal control (Gossypium hirsutum polyubiquitin protein gene, Gbp) are listed in Table S1.

Free full text: Click here

Wang L., Suo X., Liu Y., Liu C, & Luo M. (2021). Sphingosine Promotes Embryo Biomass in Upland Cotton: A Biochemical and Transcriptomic Analysis. Biomolecules, 11(4), 525.

Publication 2021

Prime Script™ RT Reagent Kit with gDNA Eraser 2xTaq Plus Master Mix (Dye Plus)

Biological Cdnas Gene Gossypium hirsutum Polyubiquitin protein Primers

Corresponding Organization : Southwest University

Other organizations : Cotton Research Institute, Chinese Academy of Agricultural Sciences

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression levels of selected genes

control variables

Gossypium hirsutum polyubiquitin protein gene (Gbp) used as internal control

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!