Seven flies were homogenized in ice-cold PBS and glucose and glycogen contents (N = 4–5) were measured using a Glucose Oxidase Assay kit (Sigma-Aldrich®), according to the manufacturer’s protocol at 540 nm. Glycogen was determined by measuring glycogen + glucose content in presence of amyloglucosidase and subtracting the glucose concentration (samples without amyloglucosidase) as described elsewhere46 (link). Glucose and glycogen contents are expressed as means of μg of glucose or glycogen per mg of proteins ± s.e.m.
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