Antimicrobial Susceptibility Testing Methodology

Full details of the susceptibility testing methodology were published previously (13 (link), 14 (link)). It was complemented by testing with tigecycline, minocycline, and trimethoprim-sulfamethoxazole at Shionogi & Co. (Osaka, Japan) using CLSI standard methods. Cation-adjusted Mueller-Hinton broth (CAMHB) (Becton, Dickinson, Sparks, MD, USA) was used for all antimicrobial susceptibility testing. For cefiderocol, iron-depleted CAMHB (ID-CAMHB) (at a final iron concentration of ≤0.03 μg/ml) was used and was prepared according to the CLSI Subcommittee on Antimicrobial Susceptibility Testing-approved methodology (14 (link)).