Synthetic Staphylococcal Matrix Assembly

The assembly reaction of the synthetic staphylococcal matrix, starting at pH 7.2, was recorded by measuring the OD₆₀₀ as a function of time every 30 s for 30 min (35 (link)). Molecular self-assembly reactions were calculated to a final volume of 4 ml, with 0.3% chitosan (medium molecular weight, 75 to 85% deacetylation), 0.15% bovine serum albumin, and 0.015% lambda DNA (all from Sigma) in TSB. The concentration (μM) of tested peptides was calculated for a final volume of 4 ml. Before getting the assembly reaction pH starting points, a calibration record was done using the same reactional tube containing all reagents (auto zero). Acetic acid and NaOH were used to adjust pH, and the reaction temperature was about 30°C. As a negative control, a similarly sized peptide was used, PA-1 (62 (link)).