Laser Capture Microdissection of Glomeruli

Laser capture microdissection was performed using the laser microdissection system LEICA AS LMD (Leica Microsystems, Wetzlar, Germany) (41 (link)) containing a VSL-337ND-s Nitrogen Laser. FFPE renal samples were used to obtain 5, 10, and 15 glomeruli. These glomeruli were laser microdissected from 3 μm sections and pressure catapulted into a tube cap (AdhesiveCap 500 clear, Zeiss). Glomeruli with severe morphological damage were excluded as the objective was to investigate the minimal amount of tissue to obtain good quality protein. The total volume of dissected glomerular tissue ranged from 7.8 to 12.5 μL. Dissected FFPE tissue was stored at −20°C until deparaffinization. All analysis were performed in triplicate.