Immunofluorescence Labeling of Tissue Sections

Immunohistochemistry and 5-bromo-4-chloro-3-indoyl-D-galactopyranoside (X-gal) staining were performed as previously described [20 (link), 41 ]. Tissues were sliced into to 12 or 20 μm thick sections. The following primary antibodies were used to perform immunofluorescence labeling: mouse anti-CRE (Millipore, 69050-3), rabbit anti-EBF1 (Merck, AB10523), rabbit anti-ISL1 (Abcam, ab20670), chicken anti-GFP (AVES Labs, GFP-1020), rat anti-BCL11B (Abcam, ab18465), rabbit anti-FOXP1 (Abcam, ab16645) and rabbit anti-SP9 (1:500) [20 (link)]. Alexa Fluor 488-, Cy3- or 647-conjugated secondary antibodies were purchased from Jackson ImmunoResearch.

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Li Z., Shang Z., Sun M., Jiang X., Tian Y., Yang L., Wang Z., Su Z., Liu G., li X., You Y., Yang Z., Xu Z, & Zhang Z. (2022). Transcription factor Sp9 is a negative regulator of D1-type MSN development. Cell Death Discovery, 8, 301.

Publication 2022

AB10523 GFP-1020 ab18465 ab16645

Alexa fluor 488 Antibodies Aves Chicken Galactopyranoside Immunofluorescence Immunohistochemistry Mouse Rabbit Tissues X gal

Corresponding Organization :

Other organizations : Children's Hospital of Fudan University

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Variable analysis

independent variables

Tissue slice thickness (12 or 20 μm)

dependent variables

Immunofluorescence labeling of CRE, EBF1, ISL1, GFP, BCL11B, FOXP1, SP9

control variables

Antibodies used for immunofluorescence labeling (mouse anti-CRE, rabbit anti-EBF1, rabbit anti-ISL1, chicken anti-GFP, rat anti-BCL11B, rabbit anti-FOXP1, rabbit anti-SP9)
Secondary antibodies used (Alexa Fluor 488, Cy3, or 647-conjugated)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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