Sandwich ELISA for DAdV-3 Detection
Corresponding Organization : Yangzhou University
Variable analysis
- Concentration of purified mAb 3D9 coated onto the ELISA plate (1.25 µg/mL)
- Absorbance values at 450 nm
- Coating of purified mAb 3D9 onto the ELISA plate at 4 °C overnight
- Blocking the ELISA plate with 5% skim milk in PBST for 2 h at 37 °C
- Incubation of virus supernatants or samples for 1 h at 37 °C
- Incubation of HRP-conjugated mAb 3D9 for 30 min at 37 °C
- Incubation with TMB substrate for 15 min at 37 °C
- Addition of 2 M H2SO4 to stop the chromogenic reaction
- Not explicitly mentioned
- Not explicitly mentioned
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