Mouse Auditory Nerve Development Transcriptome

A microarray dataset of mouse auditory nerve development from our group (NCBI Gene Expression Omnibus accession GSE59417; Lang et al., 2015 (link)) was used for comparative analysis. The dataset contains expression data for auditory nerve samples collected at P0, 3, 7, 10, 14 and 21 analyzed by Mouse 430 2.0 GeneChip (Affymetrix, Santa Clara, CA, USA). Raw hybridization data was normalized independently by both Robust Multi-array Average and MicroArray Suite 5.0 algorithms using Expression Console Software (Affymetrix). Differential expression was defined as absolute signal log ratio >0.5, ≥50% present gene detection scores and p ≤ 0.05 (Student’s t-test, unpaired) for P7 vs. P0 (identifying 2391 probe sets). False discovery rate was estimated at 1.7% based on iterative comparisons involving randomized sample group assignments. Among the 2391 probe sets, 1878 analysis-ready genes were found. Functional analysis and canonical function identification was assessed with Ingenuity Pathway Analysis Software version (IPA^®; QIAGEN, Redwood City, CA, USA).