Immunohistochemical Analysis of sLex in Human Lung

Human lung sections were immunolabeled for sLe^x as described,^{53 (link)} with an antibody directed against CD15s (BD Pharmingen; CSLEX1) and ultraView DAB (Ventana Medical Systems).^{54 (link)}

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Fonseca K.L., Maceiras A.R., Matos R., Simoes-Costa L., Sousa J., Cá B., Barros L., Fernandes A.I., Mereiter S., Reis R., Gomes J., Tapia G., Rodríguez-Martínez P., Martín-Céspedes M., Vashakidze S., Gogishvili S., Nikolaishvili K., Appelberg R., Gärtner F., Rodrigues P.N., Vilaplana C., Reis C.A., Magalhães A, & Saraiva M. (2020). Deficiency in the glycosyltransferase Gcnt1 increases susceptibility to tuberculosis through a mechanism involving neutrophils. Mucosal Immunology, 13(5), 836-848.

Publication 2020

CSLEX1 ultraView DAB

Antibody Human Lung

Corresponding Organization :

Other organizations : i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, National Center for Tuberculosis and Lung Disease, Universidade do Porto, Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Centro de Investigación Biomédica en Red de Enfermedades Respiratorias

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Variable analysis

independent variables

Immunolabeling of human lung sections for sLex

dependent variables

Expression of CD15s (sLex) in human lung sections

control variables

Immunolabeling procedure as described in reference 53
Use of an antibody directed against CD15s (CSLEX1)
Use of ultraView DAB detection system (Ventana Medical Systems) as described in reference 54

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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