Lentiviral Constructs for p190A and E-cadherin

All p190A expression constructs were synthesized with an N-terminal Myc-tag by Gene Oracle, Inc. and cloned into the lentiviral vector pUltra-hot from Addgene, plasmid #24130. The entire cDNAs for wild-type or mutant p190A forms were verified by Sanger sequencing. Validated pLKO.1 puro shRNA E-cadherin lentiviral plasmid was purchased from Addgene, plasmid #18801. Validated pLenti-EmGFP-LATS2/1-kd plasmid to deplete LATS1 and LATS2 together was likewise obtained from Addgene, plasmid #52085. Lentiviral expression construct encoding mouse E-cadherin was similarly purchased from Addgene, plasmid #18804. Lentiviral pZIP vectors encoding shRNAs targeting human p190A were purchased from transOMIC technologies Inc; cat. no. TRHS1000-35 (ARHGAP35/p190A) and validated previously [39 (link)].