Serum Lipid and Cytokine Profile Analysis

Levels of triglyceride, total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) were measured using enzymatic assay kits in the UCLA Cardiovascular Core Facility [17 (link)]. The whole mice’s blood was collected with cardiac puncture. The serum was separated from the blood for the detection of pro-inflammatory cytokines using the Quantibody Mouse Cytokine Array kit (RayBiotech, Inc., QAM-CYT-1, Peachtree Corners, GA, USA), which allows the determination of low levels of TNF-α, IL-1β, IL-6, and MCSF from the serum samples.

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Kim S.Y., Kim Y.J., Kim S., Momeni M., Lee A., Treanor A., Kim S., Kim R.H, & Park N.H. (2023). GV1001 Inhibits the Severity of the Ligature-Induced Periodontitis and the Vascular Lipid Deposition Associated with the Periodontitis in Mice. International Journal of Molecular Sciences, 24(16), 12566.

Publication 2023

Quantibody Mouse Cytokine Array kit

Blood Cardiac Cardiovascular Cholesterol Cytokine Enzymatic assay High density lipoprotein Il 1β Inflammatory Low density lipoprotein Mcsf Mouse Puncture Serum Tnf α Triglyceride Very low density lipoprotein

Corresponding Organization : University of California, Los Angeles

Other organizations : Seoul National University Dental Hospital, Polaroid (United States)

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Levels of triglyceride
Total cholesterol
High-density lipoprotein (HDL)
Low-density lipoprotein (LDL)
Very low-density lipoprotein (VLDL)
Pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, MCSF)

control variables

Not explicitly mentioned

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

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