The four lines of pea (Pisum sativum L.) seeds (JI 116, JI 2822, JI 1184 and JI 3020) in this study were selected based on the presence of specific alleles at the Def locus, which control the detachment of the seed from the funicle [4 (link)]. Two WTs and two def mutant pea seeds were kindly supplied from the John Innes Pisum Collection (Table 1 ). Tall WT (JI 116) and dwarf WT (JI 2822) have a normal abscission of seeds. Tall def mutant (JI 1184) and dwarf def mutant (JI 3020) both lack the seed abscission. The seeds of each line were sown in pots with fertilised peat and grown under greenhouse conditions at 22 °C and 16/8 h photoperiod with a photon flux of 110 μmol m−2 s−1 (400–700 nm photosynthetic active radiation) and a day length extending light provided from incandescent lamps (OSRAM, Germany).
Details of Pisum sativum L. accessions and their allelic status with respect to the Def locus
| Accession | Name | Def allele | Phenotype |
|---|---|---|---|
| JI 116 | cv. Parvus | Def (wild type) | Tall |
| JI 2822 | RIL, research line | Def (wild type) | Dwarf |
| JI 1184 | Priekuskij-341-def | def (mutant) | Tall |
| JI 3020 | cv. Nord | def (mutant) | Dwarf |
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