Establishment and Culture of Cancer Cell Lines

KPC-1 and KPC-2 cell lines^{70 (link)} were obtained from pancreas tissue from p48^creTrp53^LSL-R172HKras^LSL-G12D mice^{71 (link)}. Pan02^{72 (link),73 (link)} (DCTD Tumour Repository) cells were provided by D. Lyden. The Colon adenocarcinoma cell line MC38^{73 (link),74 (link)} and melanoma cell line B16F10^{75 (link)} (ATCC, CRL-6475 was a gift from J. D. Wolchok). Lewis lung carcinoma line LLC1 cells^{76 (link)} (ATCC, CRL-1642) were purchased from ATCC. KPC-1, KPC-2 and PANC-1 cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum, 100 U ml⁻¹ penicillin and 100 μg ml⁻¹ streptomycin (Invitrogen). Panc02 cells, LLC1 cells, MC38 cells and B16F10 cells were cultured in RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum, 100 U ml⁻¹ penicillin and 100 μg ml⁻¹ streptomycin (Invitrogen) and incubated at 37 °C in 5% CO₂.