Laser-Induced Ocular Inflammation in Mice

Male and female 10–12 week old mice underwent laser treatment as previously described^{23 (link)}. Briefly, mice were anesthetized with ketamine/xylazine (Akorn, Lake Forest, IL) and received 1 mg/kg subcutaneous injection of Meloxicam (Henry Schein Animal Health, Melville, NY). Eyes were anesthetized, dilated, and a cover slip was coupled to the cornea with Gonak (Akorn) for slit lamp biomicroscopy and laser. Four (immunofluorescence) or eight (flow cytometry, to increase inflammatory cell numbers) focal burns (75 μm, 100–120 mW, 100 ms) were administered in each eye using a 532 nm argon ophthalmic laser (IRIDEX, Mountain View, CA) via a slit lamp (Zeiss, Oberkochen, Germany).