Characterization of ESBL-Producing Enterobacterales

Irrigation water and fresh produce samples from spinach production systems were collected and ESBL-producing Enterobacterales were isolated as described (Richter et al., 2020 (link)). A selection of 19 isolates were further characterized (Table 1). The genomic DNA of each isolate was extracted with the DNeasy PowerSoil kit (Qiagen, South Africa) according to the manufacturer’s instructions. Following gDNA extraction, the concentrations were determined using the Qubit dsDNA Broad Range Assay and a Qubit 2.0 fluorometer (Life Technologies, Johannesburg) and quantification was determined on a Nanodrop 2000 (ThermoScientific, Johannesburg).

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Richter L., du Plessis E.M., Duvenage S., Allam M., Ismail A, & Korsten L. (2021). Whole Genome Sequencing of Extended-Spectrum- and AmpC- β-Lactamase-Positive Enterobacterales Isolated From Spinach Production in Gauteng Province, South Africa. Frontiers in Microbiology, 12, 734649.

Publication 2021

DNeasy PowerSoil kit Qubit dsDNA Broad Range Assay Qubit 2.0 fluorometer Nanodrop 2000

Assay Dsdna Genomic Spinach

Corresponding Organization : National Research Foundation

Other organizations : National Health Laboratory Service

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Variable analysis

independent variables

Irrigation water and fresh produce samples from spinach production systems

dependent variables

ESBL-producing Enterobacterales isolated from the samples

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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