Profiling Endocannabinoid Metabolism Modulators

2-AG, anandamide (AEA), PGE₂-G, PGE₂, and their deuterated analogs 2-AG-d5, AEA-d8, PGE₂-G-d5, and PGE₂-d4 were purchased from Cayman Chemical; stock solutions were prepared in ethanol (10 mg/mL). JZL184, a selective inhibitor of monoacylglyerol lipase, and KT182, an inhibitor of ABHD6,^{31 (link)} were purchased from Cayman Chemical. KT109, an inhibitor of DAGLβ,^{29 (link),30 (link)} and KT195, an inhibitor of serine hydrolase ABHD6,^{29 (link),30 (link)} were purchased from Sigma-Aldrich. Stock solutions of enzyme inhibitors were prepared in dimethyl sulfoxide (DMSO, 10 mg/mL) and diluted to their final concentration in sterile saline with Tween 80.