Sphere Formation Assay for Chemoresistant Cells

The sphere formation assay was performed as previously described [23 (link)]. The parental and chemoresistant cells were maintained in sphere-forming medium consisting of DMEM/F12 supplemented with N2 (Invitrogen) and B27 (Invitrogen) supplements, human recombinant epidermal growth factor (20 ng/mL), and basic fibroblastic growth factor (20 ng/mL) (PeproTech Inc., Rocky Hill, NJ, USA). They were seeded at a density of 200 cells/well in 24-well ultra-low attachment plates, and spheres that arose after 2 weeks were recorded and imaged under a light microscope.

Free full text: Click here

Gowrikumar S., Primeaux M., Pravoverov K., Wu C., Szeglin B.C., Sauvé C.E., Thapa I., Bastola D., Chen X.S., Smith J.J., Singh A.B, & Dhawan P. (2021). A Claudin-Based Molecular Signature Identifies High-Risk, Chemoresistant Colorectal Cancer Patients. Cells, 10(9), 2211.

Publication 2021

human recombinant epidermal growth factor basic fibroblastic growth factor

Assay Basic fibroblastic growth factor Cells Epidermal growth factor Human Microscope light Parental Supplements

Corresponding Organization : VA Nebraska Western Iowa Health Care System

Other organizations : Memorial Sloan Kettering Cancer Center, Albert Einstein College of Medicine, University of Nebraska at Omaha, University of Miami, Sylvester Comprehensive Cancer Center

Top 5 similar protocols

Variable analysis

independent variables

Parental cells
Chemoresistant cells

dependent variables

Sphere formation

control variables

Sphere-forming medium (DMEM/F12 supplemented with N2 and B27 supplements, human recombinant epidermal growth factor (20 ng/mL), and basic fibroblastic growth factor (20 ng/mL))
Seeding density (200 cells/well in 24-well ultra-low attachment plates)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!