To target lung metastasis, 0.5 nmol DSAB-HK or DSAB was intravenously injected into 4T1-fLuc lung metastatic mice or normal BALB/c mice (n = 5 per group). At 1, 2, 4, and 8 h postinjection, mice were subjected to NIRF imaging using an IVIS small-animal imaging system. For bioluminescence imaging (BLI), mice received an intraperitoneal injection of 150 mg/kg D-luciferin (Gold BioTechnology, St Louis, MO), and then BLI was performed using the IVIS spectrum system 10 min after D-luciferin administration. After BLI, mice were sacrificed, and the lungs were harvested, placed on a black paper, and then scanned by NIRF imaging to determine the location of DSAB-HK or DSAB.
Following the last NIRF scan at 8 h postinjection, mice were sacrificed and the major organs/tissues of mice were dissected, weighed, and NIRF imaged ex vivo. The organ uptake of DSAB-HK and DSAB were calculated as the percent injected dose per gram (%ID/g) as previously described 26 (link).
Following the last NIRF scan at 8 h postinjection, mice were sacrificed and the major organs/tissues of mice were dissected, weighed, and NIRF imaged ex vivo. The organ uptake of DSAB-HK and DSAB were calculated as the percent injected dose per gram (%ID/g) as previously described 26 (link).
