Imaging and Statistical Analysis of Plant Root and Rosette

At the end of each experiment, Petri dishes were scanned (Epson Perfection V600 Photo Scanner), and the images were analyzed using image analysis. The primary root length and rosette areas were calculated using the Image J software (Poupin et al., 2016 (link)). The root area and the non-green rosette area were calculated using the Adobe Photoshop software as described in Pinedo et al. (2015) (link). When the experiments considered two factors (bacteria and nutrients), two-way ANOVA was used, followed by a multiple comparisons test. When data was not parametric, Kruskal-Wallis or Mann-Whitney tests were performed to compare subgroups of each data (i.e., comparing bacterial treatments and the non-inoculated group under the same nutritional treatment). The replicate number is indicated in each experiment.