As previously described,[15 (link)] flow cytometry (FCM) was used to analyze cell cycle and apoptosis. The cell cycle was analyzed by ethanol-fixed cells stained with propidium iodide (PI) in buffer containing RNase A (Cat: FXP0211, 4A Biotech Co., Ltd, Beijing, China). The DNA content was assessed with a FACS Calibur flow cytometer (BD Biosciences, USA). Cell apoptosis was detected using the Annexin V-FITC Apoptosis Detection Kit (Cat: FXP022, 4A Biotech Co., Ltd, Beijing, China). The percentage of apoptotic cells were calculated with CellQuest 6.0 (BD Biosciences, USA).
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Cell Cycle and Apoptosis Analysis by Flow Cytometry
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Corresponding Organization : Air Force Medical University
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Variable analysis
independent variables
- Experimental condition (e.g., treatment or control)
- Cell cycle distribution
- Percentage of apoptotic cells
- Ethanol fixation of cells
- Staining with propidium iodide (PI) in buffer containing RNase A
- Use of FACS Calibur flow cytometer (BD Biosciences, USA)
- Use of Annexin V-FITC Apoptosis Detection Kit (Cat: FXP022, 4A Biotech Co., Ltd, Beijing, China)
- Analysis with CellQuest 6.0 (BD Biosciences, USA)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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