Macrophage Apoptosis Modulation by MoxR1

In a 24-well plate, RAW264.7 (0.5 × 10⁶) macrophage cells were plated. 4 µg/ml of MoxR1 was used to treat cells for 24 hrs at 37 °C. A positive control involving RAW264.7 cells was treated with 0.1 µM staurosporine (Sigma, USA) for 24 hrs. The ZVAD-FMK (20 µM) was used as a caspase inhibitor and served as the control for apoptosis inhibition. Untreated cells and cells treated with HI MoxR1 (4 µg/ml) served as the negative controls. The Annexin V/PI kit was used in detecting apoptosis. BD FACS Verse Flow Cytometer was used to measure the fluorescent intensity of the stained samples. Following sample reading acquisition, cells were examined using the FlowJo software.