A scheme of the UV tolerance test for characterizing the survival of diploid E. coli is illustrated in Figure 6. Diploid and wild‐type haploid E. coli cells in the exponential phase were diluted and spread on LB plates with and without antibiotics. The cells were diluted by 102‐fold for exposing to UV radiation in a sterile cabinet, and the reference without UV exposure was diluted 105‐fold. The SRs of the two strains were calculated using Equation (2).
2SR=N1/N2×103% where N1 and N2 represented the number of clones with and without UV exposure, respectively. The experiments were done in triplicate.
Free full text: Click here