Murine and Human LUAD Cell Lines for Cancer Research

The murine LUAD cell lines used and their derivations include LKR10 and LKR13 (Kras^LA1) (53 (link)); 713P, 307P, 412P, 393LN, 531P1, 531LN1, 344P, 344SQ, 531LN2, 344LN, 531LN3, and 531P2 (Kras^G12D; Trp53^R172H) (54 (link)); and KC2 and KC4 (Kras^G12D; CDKN2A^−/−) (55 (link)). Human LUAD cell lines (A549, H1299, H322, H460, H1650, H522, H596, H23, H1792, H441, H358, H157, H226, H2122, CALU-1, CALU-6, H292, and H520) were purchased (American Type Culture Collection). Murine and human LUAD cells were cultured in RPMI 1640 containing 10% fetal bovine serum. Cells were maintained at 37°C in an incubator with a humidified atmosphere containing 5% CO₂. Cells were transfected with jetPRIME Versatile DNA/siRNA transfection reagent (Polyplus). Stable cell transfectants were selected using puromycin (for pLVX, pBabe-puro, and pLKO.1 vectors) or G418 (for pcDNA3.1 and pEGFP-C3 vectors). CRISPR-Cas9–mediated p53 KO 344SQ cells were generated in the Cell-Based Assay Screening Service Core Facility (Baylor College of Medicine) using the following guide RNA sequences: 5′-acagccatcacctcactgca (tgg) and 5′-(ccc) agccactccatggcccctgt. CRISPR-Cas9–mediated p53 KO A549 cells were generated as described (13 ).