Immunohistochemical Analysis of AGR2 in HCC

The use of archived formalin-fixed, paraffin-embedded tissue blocks was approved by the Institutional Review Board of National Cheng Kung University Hospital. Tissue slides from HCC patients were evaluated via immunohistochemistry and hematoxylin/eosin staining using a polyclonal antibody against AGR2 (GeneTex, Hsinchu, Taiwan) according to the avidin–biotin complex method, as described previously [31 (link)]. Immunoreactivity for AGR2 was visualized using DAB/nickel substrate (Vector Laboratories, Burlingame, CA).

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Tsai H.W., Chen Y.L., Wang C.I., Hsieh C., Lin Y.H., Chu P.M., Wu Y.H., Huang Y.C, & Chen C.Y. (2023). Anterior gradient 2 induces resistance to sorafenib via endoplasmic reticulum stress regulation in hepatocellular carcinoma. Cancer Cell International, 23, 42.

Publication 2023

DAB/nickel substrate

Antibody Avidin Biotin Embedded paraffin Eosin Formalin Immunohistochemistry Institutional review board Nickel Patients Tissue Vector

Corresponding Organization :

Other organizations : National Cheng Kung University Hospital, National Cheng Kung University, China Medical University, Chiayi Chang Gung Memorial Hospital, Chang Gung Memorial Hospital, Chung Shan Medical University

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Variable analysis

independent variables

Use of archived formalin-fixed, paraffin-embedded tissue blocks

dependent variables

Immunoreactivity for AGR2 using DAB/nickel substrate

control variables

Tissue slides from HCC patients
Evaluation via immunohistochemistry and hematoxylin/eosin staining
Use of a polyclonal antibody against AGR2 (GeneTex, Hsinchu, Taiwan) according to the avidin–biotin complex method

controls

Positive control: Not specified
Negative control: Not specified

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