Anti-HA-Tag antibody (sc-7392; Santa Cruz, Dallas, TX) was used to detect TMPRSS2 and anti-c-Myc antibody (sc-40, Santa Cruz) was used to detect furin, while anti-ACE2 (10108-T60; Sino Biological Inc., Beijing, China) and anti-CTSL (10486-RP02 Sino Biological Inc.) antibodies were used to detect ACE2 and CTSL, respectively. Anti-S2 antibody against SARS-CoV-2 S protein was generated in-house by immunizing mice with recombinant S2 protein. The anti-VSV M protein antibody was purchased from KeraFast (EB0011; Boston, MA). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (CW0102S; CWbiotech, Beijing, China) and HRP-conjugated goat anti-rabbit IgG (SSA004; Sino Biological Inc.) were used as secondary antibodies. Nine monoclonal antibodies against SARS-CoV-2 S protein were used in the neutralization assays, of which CB6 and CA1 were gifts from Dr. Jinghua Yan of University of Chinese Academy of Sciences [51 (link)]; X593 was from Dr. X. Sunney Xie of Peking University [52 (link)]; H014 and H00S002 were from Dr. Liangzhi Xie of Sino Biological Company [53 (link)]; 1F11, A261–262, A157, and A247 were from Dr. Linqi Zhang of Tsinghua University [54 (link)]. Furin inhibitor (Decanoyl-RVKR-CMK) was from R&D Systems (Bristol, UK, Cat#: 3501) Cathepsin inhibitor (E64D) was from Apexbio (Shanghai, China, Cat#: A1903).
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