Isolation and culture of ovarian cancer cells

Sample collection was performed with patient consent and approved by the Human Investigation Committee of Yale University School of Medicine. Ovarian cancer cells were isolated from patients diagnosed with Stage III/IV serous ovarian carcinoma. These in-house derived ovarian cancer cells (R182, R2615, 01-28, and Tara R182) have been previously described [10 (link),11 (link),12 (link),13 (link),14 (link),15 (link)]. Ovarian cancer cell lines (OVCAR3, OVCA432, SKOV3, and A2780) were purchased from ATCC. Cells were cultured in RPMI media supplemented in 10% FBS and grown at 37 °C with 5% CO₂. All of the cell lines tested negative for mycoplasma, authenticated once a year by STR profiling, and used within six passages between experiments.