Total protein extracts (50 μg) were subjected to SDS-PAGE as described [29 (link)]. After blocking, proteins were probed with anti-PPARγ (PA3-821A), anti-phosphatase and tensin homolog PTEN (sc25778) and anti-β-Actin (sc69879) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies, overnight, and images were acquired using Odyssey FC (Licor, Lincoln, NB, USA).
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