Identification and Analysis of ZjRNase Genes

The genome sequences of Ziziphus jujuba Mill. were obtained from the National Center for Biotechnology Information (NCBI) database [19 (link)]. The sequences of 14, 9 identified MdRNases, PyRNases were downloaded from the National Center for Biotechnology Information (NCBI) database. In addition, the sequences of8 identified OsRNases were downloaded [1 (link)]. RNases were identified by two rounds of BLASTP searches. First, the sequences of all OsRNases were used to search for possible ZjRNases sequences via TBtools [36 (link)]. Then, NCBI Batch CD-Search was used to confirm whether the candidate RNases contained the RNase_T2 superfamily domain (pfam00445) or the Ribonuclease_T2 domain (cl00208). A total of 4 ZjRNase genes were ultimately identified in the genome. The protein length, isoelectric point (pI) and molecular weight (MW) were subsequently predicted.