Investigating HCMV-induced SOCS3 expression

NPCs were seeded on poly-D-lysine-coated coverslips in 12-well plates and infected with HCMV after attachment. The coverslips were harvested and fixed with 4% paraformaldehyde at the indicated times post-infection. PBS-perfused Brains were fixed in 4% paraformaldehyde and prepared for IFA. anti-SOCS3 (IgG2b; ab14939, Abcam), anti-SOCS3 (Rabbit; ab16030, Abcam), anti-HA (Rabbit; Covance) and anti-IE1/2 (IgG1; Virusys) were detected by primary antibodies and appropriate secondary antibodies [35 (link),64 (link)]. The secondary antibodies included TRITC-mouse IgG2b (1090–03, Southern Biotech), Alexa Fluor 488-mouse IgG1 (A-21121, Invitrogen), Alexa Fluor 647-rabbit (A-21247, Invitrogen), and Nuclei were counterstained with DAPI (4′,6-diamidino-2- phenylindole) (D9542, Merck).

Free full text: Click here

Wang X.Z., Wen L., Zhou Y.P., Huang S.N., Yang B., Cheng S., Zeng W.B., Mei M.J., Sun J.Y., Jiang X., Cheng H, & Luo M.H. (2023). Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells. PLOS Pathogens, 19(2), e1011166.

Publication 2023

ab14939 ab16030 D9542

Alexa fluor 488 Alexa fluor 647 Antibodies Brains Dapi Hcmv Igg1 Igg2b Infection Lysine Mouse Nuclei Paraformaldehyde Poly Rabbit Tritc

Corresponding Organization : Shanghai Public Health Clinical Center

Top 5 similar protocols

Variable analysis

independent variables

HCMV infection

dependent variables

SOCS3 expression
HA expression
IE1/2 expression

control variables

NPCs seeded on poly-D-lysine-coated coverslips
Paraformaldehyde fixation
PBS-perfused brain tissue preparation
Primary antibodies: anti-SOCS3 (IgG2b), anti-SOCS3 (Rabbit), anti-HA (Rabbit), anti-IE1/2 (IgG1)
Secondary antibodies: TRITC-mouse IgG2b, Alexa Fluor 488-mouse IgG1, Alexa Fluor 647-rabbit
DAPI nuclear staining

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!