Extraction and Characterization of Chimonanthus nitens Oliv Polysaccharides

COP1 was obtained in the same way as previously reported [28 ]. In general, Chimonanthus nitens Oliv leaves were dried, crushed and sifted, soaked in petroleum ether to remove the fat soluble substance in the powder, and dried for use. Distilled water was added to the powder at 20 : 1 (v/w) and extracted for 2 h in the ultrasonic cycle at 85°C. The filtrate was collected and concentrated with a rotary evaporator. Four volumes of 95% ethanol were added to the concentrated liquid, and precipitates were collected. The precipitate was dissolved in distilled water, the alcohol was removed, and the protein was removed by Sevag method. Sevag reagent (chloroform : n-butanol = 4 : 1, v/v) was mixed with polysaccharide solution (3 : 1, v/v), shaken for 20 min, and then centrifuged. The concentrated polysaccharide solution was depressurized in vacuum and freeze-dried to obtain crude polysaccharides (COP). COP was adsorbed by DEAE cellulose column and eluted with 0.1 M NaCl solution. The eluent was collected, and the component was named COP1 after being dialyzed with tap water for 48 h, distilled water for 24 h, concentration, and lyophilization. Its carbohydrate, uronic acid, and protein contents were 83.15 ± 1.17%, 10.36 ± 0.54%, and 1.61 ± 0.36%, respectively. COP1 was 18.843 kDa and mainly consisted of Glu, Xyl, Gla, and Ara, with percentage of moles 7.4 : 11.1 : 24.9 : 56.6 [28 ].