Extraction of Absolute from CS Flowers

CS samples were obtained from the field near Hanaro Farm (Haenam County, Jeollanam-do, Republic of Korea) (34°23′00.4″ N 126°33′59.0″ E; 25 April 2019). CS identity was confirmed by Jong-Cheol Yang from the Division of Forest Biodiversity and Herbarium, Korea National Arboretum (Republic of Korea). A voucher specimen (no. CS-0001) was kept at the Herbarium of the College of Life and Health Science, Hoseo University (Republic of Korea). Absolute was extracted from CS flowers by solvent extraction as previously described [27 (link)]. In brief, flowers (5.27 kg) were completely immersed in hexane (Samchun, Pyeongtaek, Republic of Korea) at room temperature (RT) for 1 h. Solvent was removed from extracts using a rotary evaporator (EYELA, Tokyo, Japan) at 25 °C under vacuum to yield a dark yellow waxy residue (concrete), which was mixed with ethanol and left at −20 °C overnight. The mixture was then filtered through a sintered glass funnel, and the ethanol was evaporated at 35 °C. The light yellow wax obtained (absolute) (CSFAb; 1.50 g, yield 0.028%, w/w) was stored at −80 °C until required.