MCF-7 and Fibroblast Cell Culture

MCF-7 cells (HTB-22) were obtained from the American Type Culture Collection (Manassas, Rockville). Cells were grown as monolayer cultures at 37°C and 5% CO₂, in Eagle's minimal essential medium (E'MEM) supplemented with 10% (v/v) FBS (Sigma), 2 mM L-glutamine (Sigma), 20 mM HEPES (Sigma), 0.025 μg/ml amphotericin B (Sigma), 100 IU/ml penicillin G (Sigma), and 100 μg/ml streptomycin (Sigma). Primary fibroblasts were isolated and identified as previously described [34 (link)]. The donor donating tissues gave written informed consent, and this study was approved by the Ethical Committee for Biomedical Research of the Vietnam National University, Ho Chi Minh City (Ref. 1387 QĐ-ĐHQG). Cells were cultured in D'MEM/F12 supplemented with 10% (v/v) FBS, 20 mM HEPES, 0.025 μg/ml amphotericin B, 100 IU/ml penicillin G, and 100 μg/ml streptomycin at 37°C, 5% CO₂.