Protein Expression Analysis by Western Blot

Protein extraction and western blot were performed as previously described(68 (link)). Antibodies for FOXN4 (#PA539174, ThermoFisher), ONECUT2 (#ab28466, Abcam), POU3F2 (#12137, Cell Signaling Technology), EGFR (#4267, Cell Signaling Technology), ASCL1 (#556604, BD), NEUROD1 (#ab109224, Abcam), pAKT (S473, #4060S, Cell Signaling Technology), pPRAS40 (T246, #13175, Cell Signaling Technology), Beta catenin (#8480, Cell Signaling Technology), Vinculin (#13901, Cell Signaling Technology) and actin (#3700, Cell Signaling Technology) were used.

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Quintanal-Villalonga A., Taniguchi H., Zhan Y.A., Hasan M.M., Chavan S.S., Meng F., Uddin F., Manoj P., Donoghue M.T., Won H.H., Chan J.M., Ciampricotti M., Chow A., Offin M., Chang J.C., Ray-Kirton J., Tischfield S.E., Egger J., Bhanot U.K., Linkov I., Asher M., Sinha S., Silber J., Iacobuzio-Donahue C.A., Roehrl M.H., Hollmann T.J., Yu H.A., Qiu J., de Stanchina E., Baine M.K., Rekhtman N., Poirier J.T., Loomis B., Koche R.P., Rudin C.M, & Sen T. (2021). Multi-omic analysis of lung tumors defines pathways activated in neuroendocrine transformation. Cancer discovery, 11(12), 3028-3047.

Publication 2021

POU3F2 ASCL1 NEUROD1 pAKT pPRAS40 Beta catenin Vinculin actin

Actin Antibodies Beta catenin Egfr Neurod1 Onecut2 Protein Vinculin Western blot

Corresponding Organization : Cornell University

Other organizations : Molecular Oncology (United States), Parker Institute for Cancer Immunotherapy, NYU Langone Health

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of FOXN4, ONECUT2, POU3F2, EGFR, ASCL1, NEUROD1, pAKT (S473), pPRAS40 (T246), Beta catenin, Vinculin, and actin

control variables

None explicitly mentioned

controls

The authors do not specify any positive or negative controls.

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