The B/Yamagata/16/88 (B/Yamagata, Yamagata lineage) virus was provided by Dr. Man Ki Song (International Vaccine Institute, Seoul, Korea). The B/Yamagata/16/88 virus was propagated in Madin-Darby canine kidney cells (ATCC CCL-34; ATCC, Manassas, VA, USA) as described previously (18 (link)19 (link)). The precipitated virus was suspended in PBS and stored at −80°C. For challenge infection, mice were lightly anesthetized with isoflurane and intranasally administered 5 to 10 LD50 of the virus.
Protocol detail
Influenza B Virus Propagation and Infection
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Ewha Womans University
Top 5 similar protocols
Variable analysis
independent variables
- Dose of B/Yamagata/16/88 virus (5 to 10 LD50) administered intranasally to mice
- Infection and health outcomes in mice after challenge with B/Yamagata/16/88 virus
- Madin-Darby canine kidney cells (ATCC CCL-34) used to propagate the B/Yamagata/16/88 virus
- Isoflurane used to lightly anesthetize the mice prior to intranasal virus administration
- None specified
- None specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!