Cell proliferation was determined according to a previously described method31 (link) and per manufacturer’s instructions (Roche, Basel, Switzerland). Cells were incubated with AgNPs for 24 h; the BrdU labeling solution was added to the culture medium 2 h before the end of the incubation. Cells were fixed and the level of incorporated BrdU was determined using the Cell Proliferation ELISA BrdU assay kit (Roche) following the manufacturer’s instructions. Proliferation activity of the untreated cells at the time point of 0 h was considered as 100%.
Protocol detail
Cell Proliferation Assay with AgNPs
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Corresponding Organization : Konkuk University
Other organizations : Yangzhou University, Kerala Veterinary and Animal Sciences University, Yichun University
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Variable analysis
independent variables
- AgNPs (silver nanoparticles)
- Cell proliferation
- Incorporated BrdU (bromodeoxyuridine) level
- Incubation time (24 h)
- BrdU labeling solution addition (2 h before the end of incubation)
- Proliferation activity of untreated cells at 0 h (considered as 100%)
- Positive control: Untreated cells at 0 h
- Negative control: Not explicitly mentioned
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