CRLF2 expression in T-ALL

To assess CRLF2 expression on the surface of T-ALL blasts the following combination of antibodies was used: CRLF2PE (Clone 1B4, Biolegend, London, UK) [24 (link)] or isotype matched IgG (Biolegend), CD45PerCP (BD Biosciences, Franklin Lakes, NJ, USA) and CD7ECD (Beckman Coulter, Brea, California, USA). Leukemic blasts were gated as CD45 intermediate/CD7+. The T-ALL cell lines were stained only with the CRLF2PE or the isotype antibody.

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Palmi C., Savino A.M., Silvestri D., Bronzini I., Cario G., Paganin M., Buldini B., Galbiati M., Muckenthaler M.U., Bugarin C., Mina P.D., Nagel S., Barisone E., Casale F., Locatelli F., Nigro L.L., Micalizzi C., Parasole R., Pession A., Putti M.C., Santoro N., Testi A.M., Ziino O., Kulozik A.E., Zimmermann M., Schrappe M., Villa A., Gaipa G., Basso G., Biondi A., Valsecchi M.G., Stanulla M., Conter V., te Kronnie G, & Cazzaniga G. (2016). CRLF2 over-expression is a poor prognostic marker in children with high risk T-cell acute lymphoblastic leukemia. Oncotarget, 7(37), 59260-59272.

Publication 2016

isotype matched IgG CD45PerCP

Antibodies Antibody Cell lines Clone Crlf2 Isotype T all

Corresponding Organization : University of Milano-Bicocca

Other organizations : University of Padua, University Hospital Schleswig-Holstein, University of Lübeck, Heidelberg University, University Hospital Heidelberg, Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, University of Naples Federico II, Bambino Gesù Children's Hospital, University of Pavia, Azienda Ospedaliero-Universitaria Policlinico - Vittorio Emanuele, Istituto Giannina Gaslini, University of Bologna, University of Bari Aldo Moro, Sapienza University of Rome, Azienda di Rilievo Nazionale ed Alta Specializzazione, Medizinische Hochschule Hannover

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Variable analysis

independent variables

CRLF2PE (Clone 1B4, Biolegend, London, UK) [24 (link)]
Isotype matched IgG (Biolegend)

dependent variables

CRLF2 expression on the surface of T-ALL blasts

control variables

CD45PerCP (BD Biosciences, Franklin Lakes, NJ, USA)
CD7ECD (Beckman Coulter, Brea, California, USA)
Leukemic blasts were gated as CD45 intermediate/CD7+

controls

Positive control: T-ALL cell lines stained with CRLF2PE
Negative control: T-ALL cell lines stained with isotype matched IgG

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