Protocol detail

RNA Isolation from Frozen Kidney Tissue

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The kidney tissues snap-frozen and stored at -80°C were used for mRNA microarray analyses as previously described [21 (link)]. Total RNA was isolated using the miRNeasy 96 kit (Qiagen, Cat No 217061, Valencia, CA), according to the manufacturer’s instructions. Quality and quantity of RNA were evaluated with a 2100 Bioanalyzer (Cat. No. 62947, Agilent Technologies, Santa Clara, CA), using the Agilent RNA 6000 Nano Reagents (Cat. No. 5067–1511, Agilent Technologies,), and a multi-well Nanodrop 8000 spectrophotometer (Cat. No. 1464, Scientific, Waltham, MA).

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Speir R.W., Stallings J.D., Andrews J.M., Gelnett M.S., Brand T.C, & Salgar S.K. (2015). Effects of Valproic Acid and Dexamethasone Administration on Early Bio-Markers and Gene Expression Profile in Acute Kidney Ischemia-Reperfusion Injury in the Rat. PLoS ONE, 10(5), e0126622.

Publication 2015

miRNeasy 96 kit Agilent RNA 6000 Nano Reagents Nanodrop 8000 spectrophotometer

Frozen Kidney Microarray analyses Mrna Tissues

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Variable analysis

independent variables

Kidney tissues snap-frozen and stored at -80°C

dependent variables

MRNA expression (analyzed by microarray)

control variables

Quality and quantity of RNA (evaluated with a 2100 Bioanalyzer and a multi-well Nanodrop 8000 spectrophotometer)

controls

No positive or negative controls were explicitly mentioned.

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